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Announcements
Subject:
Bigelow Lab Course Listings 2005
Dear Colleagues, Bigelow Laboratory is pleased to announce its course
offerings for 2005. Five courses at the upper undergraduate level/graduate
level are planned. Courses carry 2 or 3 graduate credits from the
University of New England. Applications for all courses are now
being accepted; please be sure to note the application deadline
for each course. Course participation for the courses will be limited
to 8 - 12 individuals in order to provide maximum exposure for the
student. Participants will be chosen based upon application materials
and the date the deposit is received. To register for the course
or for further information, please contact Ms Jane Gardner at the
email address below. Also see the Bigelow Laboratory for Ocean Sciences
web site at http://www.bigelow.org/course/ . Please forward this
message to any interested individuals. Thank you very much. Sincerely,
William Balch Senior Research Scientist Course Coordinator Bigelow
Laboratory for Ocean Sciences P.S. Please excuse any duplicate mailings.
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Course Offerings Phytoplankton Culture Techniques 18-24 May, 2005;
2 credit hours; application deadline 1 March 2005 Dr. Robert Andersen
and Dr. Michael Sieracki Bigelow Laboratory for Ocean Sciences The
Provasoli-Guillard National Center for Culture of Marine Phytoplankton
will offer an intensive seven-day course covering basic and advanced
techniques for isolating, growing and cryopreserving marine phytoplankton.
The course is designed for academic graduate students and faculty
members as well as aquaculturists. Isolation methods include direct
single-cell isolation by micropipette, agar plating and flow cytometry
and indirect methods such as dilution techniques and enrichments.
Preparation and sterilization of various culture media will be described.
Instructors will provide methods for culture purification, including
physical (e.g. single-cell "washing"), chemical (e.g. antibiotic
treatments) and automated (e.g., flow cytometry) approaches. Students,
in groups no larger than 5-6, will participate in flow cytometric
laboratory exercises for isolating and purifying culture strains
and in cryopreservation laboratory exercises. Cryopreservation will
include discussion of cryoprotectants, methods for freezing (simple
techniques to computer-assisted control-rate freezers) and methods
for thawing frozen cells. Other topics will include factors affecting
culture health, such as light, temperature, salinity, nutrients
and aeration. Mass culturing will also be addressed, and numerous
species from the CCMP collection will be available for study. A
copy of the book Algal Culturing Techniques will be supplied to
each student. ***********************************************************************
Microzooplankton Ecology 6-10 June, 2005; 2 credit hours; application
deadline 1 March 2005 Dr. Diane Stoecker, Horn Point Laboratory
University of Maryland Center for Environmental Science This one-week
lecture course will provide the fundamentals of microzooplankton
ecology as well as the latest techniques for their study. Topics
to be covered include: functional ecology of heterotrophic flagellates,
planktonic ciliates, heterotrophic-mixotrophic dinoflagellates,
techniques for microzooplankton culturing , observation and enumeration,
microzooplankton behavior and life history strategies, techniques
for estimation of growth and grazing rates, mixotrophy and plastid
retention, food web dynamics, impact on C, S and N cycles, and challenges
of incorporating microzooplankton into models. Practical examples
will be provided and integrated with the students' specific interests.
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Early Life History of Marine Fishes 1-17 August, 2005; 3 credit
hours; Application deadline 1 May 2005 John E. Olney, College of
William and Mary Edward D. Houde, University of Maryland Center
for Environmental Science *Course to be held at the Marine Science
Center of the University of New England, Biddeford, ME This graduate
level lecture and laboratory course offers a comprehensive view
of the biology and taxonomy of early life stages of fishes. Pelagic
eggs, larvae, and newly-transformed juveniles are abundant and diverse
components of aquatic ecosystems that are vulnerable to biotic and
abiotic stress. Morphological variation throughout ontogeny reflects
ecological roles and systematic relationships. This class is intended
for students with an interest in fish ecology, fisheries science,
ichthyology, or biological oceanography, and an appropriate background
in those disciplines is required. For further information, visit
http://www.une.edu/cas/msc/course.html. Note, to register for this
specific course only, contact Mike Dunnington at the University
of New England (mdunnington@une.edu, or 207-283-0170, ext. 2671).
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Small-Scale Physical-Biological Interactions in the Plankton 15-19
August 2005; 2 credit hours; application deadline 15 May 2005 George
Jackson, Texas A&M University Thomas Kiorboe, Danish Institute for
Fisheries Research David Fields, Bigelow Laboratory for Ocean Sciences
The adaptations of planktonic organisms, from viruses and bacteria
to larval fish, can be understood only in the context of the immediate
physical and chemical environment in which they live. Nutrient uptake,
motility patterns, feeding and encounter rates, signal transmission
and perception are all constrained by often non-intuitive interactions
between organism biology and small-scale physical and chemical characteristics
of the fluid environment (e.g. viscosity, fluid motion, diffusion).
This course will examine the life and interactions of planktonic
organisms in their small-scale world through lectures and laboratory
and computer exercises and demonstrations. ***********************************************************************
Aquatic Cytometry: Applications of Flow and Imaging Cytometry to
the Aquatic Sciences Date 12 - 16 September 2005; 2 credit hours;
application deadline 15 June 2005 Dr. Michael Sieracki and Dr. Nicole
Poulton Bigelow Laboratory for Ocean Sciences This course will cover
the application of flow and imaging cytometry techniques for the
study of marine particles with the emphasis on planktonic microorganisms
- including prokaryotes, protozoa, and phytoplankton. Emphasis will
be on the basics of flow cytometry and sorting, image analysis with
fluorescence microscopy, and imaging-in-flow technology. There are
hands-on operation opportunities with our BD FACScan, DAKO-Cytomation
Mo Flo sorter, and FlowCAM. Participants are encouraged to develop
small projects. The resources of the Center for Culture of Marine
Phytoplankton (http://ccmp.bigelow.org) and the flow and imaging
cytometry instruments here at Bigelow are available for the course
and individual projects. See http://www.bigelow.org/cytometry and
http://flowcam.bigelow.org for more information. ***********************************************************************
For more information on any of these courses, please contact Ms
Jane Gardner, Bigelow Laboratory for Ocean Sciences, POB 475, W.
Boothbay Harbor, ME. 04575; Tel. 207-633-9600; email- jgardner@bigelow.org
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