HPLC_pigments

 PI:              Ralf Goericke
 of:              University of California, San Diego
 dataset:         Pigments, HPLC method, standard casts
 project/cruise:  AESOPS/RR_KIWI_9, APFZ Process 2
 ship:            R/V Roger A. Revelle

 Methodology:
	Samples that had been frozen in liquid nitrogen were extracted as described
	previously (Goericke and Repeta, 1993, MEPS 101: 307 - 313).
	Pigment extracts were analyzed on the reverse phase HPLC system described
	below.   Pigments were detected by absorbance at 440nm.  The identity of
	pigments was confirmed by frequently checking on-line spectra against those
	of standards.  

  Column - Alltech Adsorbosphere C18, HS 3um, 4.6 mm, 10 cm.
  Solvents - A [methanol : acetonitrile : water : 0.5M aq ammonium acetate
            (30:30:30:10)
           B [methanol : acetonitrile : ethyl acetate (10:35:55)].
  Gradient - [time (min); solvent A, solvent B] - [0; 100, 0],   [4; 75, 25],
           [24; 12, 88], [26; 12, 88], [28; 100, 0]. 
  Instruments - Waters 510 pumps, Shimadzu autosampler and uv/vis detector,
         Waters 991M photodiode detector and Waters Millenium data system 
 
 Parameter       Description                               Units

 event           event number from event log
 sta             station number from event log
 cast            cast number from event log
 cast_type       TM = Trace Metal free rosette
                 CTD = CTD rosette
 bot             TM or CTD bottle number
 depth_n         nominal sample depth                      meters
 allox           alloxanthin                               nanogram/liter
 carotene_a      alpha carotene                            nanogram/liter
 carotene_b      beta carotene                             nanogram/liter
 carotene_g      gamma carotene                            nanogram/liter
 chl_a1          monovinyl chlorophyll a                   nanogram/liter
 chl_a1_prime    monovinyl chlorophyll a prime             nanogram/liter
 chl_a_tot       sum of chlorophyll a like pigments (*)    nanogram/liter
 chl_b1          monovinyl chlorophyll b                   nanogram/liter
 chl_c12         chlorophyll c12                           nanogram/liter
 chl_c3          chlorophyll c3                            nanogram/liter
 chlide_a        chlorophyllide a                          nanogram/liter
 diadinox        diadinoxanthin                            nanogram/liter
 diatox          diatoxanthin                              nanogram/liter
 fucox           fucoxanthin                               nanogram/liter
 fucoxanthiol    fucoxanthiol                              nanogram/liter
 fucox_but       19'-butanoyloxyfucoxanthin                nanogram/liter
 fucox_hex       19'-hexanoyloxyfucoxanthin                nanogram/liter
 fucox_iso1      fucoxanthin isomer 1                      nanogram/liter
 fucox_iso2      fucoxanthin isomer 2                      nanogram/liter
 lutein          lutein                                    nanogram/liter
 chl_c3_p        phytolated chlorophyll c3 (+)             nanogram/liter
 peridinin       peridinin                                 nanogram/liter
 violax          violaxanthin                              nanogram/liter
 zeax            zeaxanthin                                nanogram/liter

(*) chl_a_tot = chl_a1 + chl_al' + allochl_a1 + allochl_a2 + allochlide_a + chlide_a

(+) allochl_a1 was not sufficiently resolved from chl_c3_p