bacteria_sed

 PI:              Fred Dobbs
 of:              Old Dominion University	
 dataset:         bacteria abundance, in sediments 	
 project/cruise:  EqPac/TT013, Benthic survey		
 ship:            R/V Thomas Thompson

 Methodology:
 Samples were fixed in formaldehyde solution (final conc. 1%) and stored 
 in the dark at 4°C.  Prior to enumeration, samples were diluted, 
 filtered onto 0.2 um black polycarbonate filters, and stained with the 
 nucleic acid stain DAPI (4' 6-diamidino-2-phenylindole; final conc. 
 1 ug ml-1)  (Porter and Feig 1980).  Filters were stored in the dark at 
 -85°C until the bacteria were counted using epifluorescent microscopy. 

 DMO Notes:
 All samples are extracted from multicores.   Data are displayed as a 
 summary at a nominal geographic location.  The summary consists of 
 samples from one or more cores or stations.  Sample size is 0.5cm.  
 Cell counts are the mean of 2 samples.

  
  Parameter    Description                               Units

  sta_name     station name                              dimensionless
  sta          station number from event log             dimensionless
  lat_n        nominal latitude; negative denotes south  degrees
  lon_n        nominal longitude; negative denotes west  degrees
  tube_num     tube number, multicore                    dimensionless
  depth_core   depth in core, mid-point of interval      centimeters 
               sampled                      
  bact_cm3     bacteria abundance, in units of           cells/cubic centimeter
               sediment volume         
  bact_cm3_sd  bacteria abundance, in units of           cells/cubic centimeter 
               sediment volume, error of one standard 
               deviation
  bact_g       bacteria abundance, in units of           cells/gram sed. dry wt.
               sediment dry weight
  bact_g_sd    bacteria abundance, in units of           cells/gram sed. dry wt.
               sediment dry weight, error of one 
               standard deviation