JGOFS Protocols
on-line thanks to Beatriz Balino, JGOFS CPO
JGOFS Report Nr. 19
Table of Contents, also available in
pdf format
Preface 1
Chapter 1 Introduction by Dr. A.H. Knap 3
Chapter 2 Shipboard Sampling Procedures
1.0 Introduction 5
2.0 Hydrocasts 5
3.0 Water Sampling 6
4.0 Primary Production 6
5.0 Sediment Trap Deployment and Recovery 7
6.0 Shipboard Sample Processing 7
Chapter 3 CTD and Related Measurements
1.0 Scope and field of application 9
2.0 Apparatus 9
3.0 Data Collection 11
4.0 Data Processing 12
5.0 References 16
Chapter 4 Quality Evaluation and Intercalibration
1.0 Introduction 19
Chapter 5 Salinity Determination
1.0 Scope and field of application 21
2.0 Definition 21
3.0 Principle of Analysis 21
4.0 Apparatus 21
5.0 Reagents 21
6.0 Sampling 22
7.0 Procedures 22
8.0 Calculation and expression of results 22
9.0 Quality assurance 23
10.0 References 23
Chapter 6 Determination of Dissolved Oxygen by the Winkler
Procedure
1.0 Scope and field of application 25
2.0 Definition 25
3.0 Principle of Analysis 25
4.0 Apparatus 26
5.0 Reagents 27
6.0 Sampling 28
7.0 Tiration Procedures 29
8.0 Calculation and expression of results 31
9.0 Quality assurance 32
10.0 References 33
Chapter 7 The Determination of Total Inorganic Carbon by
the Coulometeric Procedure
1.0 Scope and field of application 35
2.0 Definition 35
3.0 Principle of Analysis 35
4.0 Apparatus 36
5.0 Reagents 37
6.0 Sampling 38
7.0 Procedures 39
8.0 Calculation and expression of results 41
9.0 Quality assurance 41
10.0 References 42
Chapter 8 The Determination of Nitrite, Nitrate +Nitrite,
Orthophosphate and Reactive Silicate in Sewater
using continuous Flow Analysis
1.0 Scope and field of application 43
2.0 Definition 43
3.0 Principle of Analysis 45
4.0 Apparatus 46
5.0 Reagents 51
6.0 Sampling 54
7.0 Procedures and Standardization 57
8.0 Analytical Methods 65
9.0 Calculations 81
10.0 Quality Assurance 88
11.0 References 90
Chapter 9 The Determination of Nitrate in Sea Water
1.0 Scope and field of application 93
2.0 Definition 93
3.0 Principle of Analysis 93
4.0 Apparatus 94
5.0 Reagents 94
6.0 Sampling 94
7.0 Procedures 95
8.0 Calculation and expression of results 97
9.0 Notes 97
10.0 References 98
Chapter 10 The Determination of Nitrite in Sea Water
1.0 Scope and field of application 99
2.0 Definition 99
3.0 Principle of Analysis 99
4.0 Apparatus 99
5.0 Reagents 99
6.0 Sampling 100
7.0 Procedures 100
8.0 Calculation and expression of results 101
9.0 References 102
Chapter 11 The Determination of Phosphorus in Sea Water
1.0 Scope and field of application 103
2.0 Definition 103
3.0 Principle of Analysis 103
4.0 Apparatus 103
5.0 Reagents 103
6.0 Sampling 104
7.0 Procedures 104
8.0 Calculation and expression of results 105
9.0 References 106
Chapter 12 The Determination of Reactive Silicate in Sea
Water
1.0 Scope and field of application 107
2.0 Definition 107
3.0 Principle of Analysis 107
4.0 Apparatus 107
5.0 Reagents 107
6.0 Sampling 108
7.0 Procedures 108
8.0 Calculation and expression of results 109
9.0 Notes 110
10.0 References 110
Chapter 13 Measurement of Algal Chlorophylls and Carotenoids
by HPLC
1.0 Scope and field of application 111
2.0 Definition 111
3.0 Principle of Analysis 111
4.0 Apparatus and Reagents 112
5.0 Eluants 113
6.0 Sample Collection and Storage 113
7.0 Procedure 113
8.0 Calculation and expression of results 115
9.0 References 115
Chapter 14 Measurement of Chlorophyll a and Phaeopigments
by Fluorometric Analysis
1.0 Scope and field of application 119
2.0 Definition 119
3.0 Principle of Analysis 119
4.0 Apparatus 119
5.0 Reagents 120
6.0 Sample Collection and Storage 120
7.0 Procedure 120
8.0 Calculation and expression of results 122
9.0 References 122
Chapter 15 Determination of Particulate Organic Carbon and
Particulate Nitrogen
1.0 Scope and field of application 123
2.0 Definition 123
3.0 Principle of Analysis 123
4.0 Apparatus 123
5.0 Reagents 124
6.0 Sampling 124
7.0 Procedures 124
8.0 Calculation and expression of results 125
9.0 References 125
Chapter 16 Determination of Dissolved Organic Carbon by a
High Temperature Combustion/Direct Injection
Technique
1.0 Scope and field of application 127
2.0 Definition 127
3.0 Principle of Analysis 127
4.0 Apparatus 128
5.0 Reagents 129
6.0 Sampling 130
7.0 Procedures 131
8.0 Calculation and expression of results 134
9.0 Quality control/quality assessment 137
10.0 Notes 139
11.0 Intercomparison 141
12.0 References 142
Chapter 17 Determination of New Production by 15N
1.0 Scope and field of application 145
2.0 Definition 145
3.0 Principle of Analysis 145
4.0 Apparatus 145
5.0 Reagents 146
6.0 Sampling 146
7.0 Procedures 146
8.0 Calculation and expression of results 147
9.0 Quality Control 148
10.0 Intercomparison 149
11.0 Parameters 149
12.0 References 149
Chapter 18 Determination of Bacterioplankton Abundance
1.0 Scope and field of application 151
2.0 Definition 151
3.0 Principle of Analysis 151
4.0 Apparatus 151
5.0 Reagents 152
6.0 Sampling 152
7.0 Procedures 152
8.0 Calculation and expression of results 153
9.0 Quality Control 153
10.0 References 154
Chapter 19 Primary Production by 14C
1.0 Scope and field of application 155
2.0 Definition 155
3.0 Principle of Analysis 155
4.0 Apparatus 156
5.0 Reagents and Supplies 156
6.0 Sampling 158
7.0 Procedures 160
8.0 Calculation and expression of results 160
9.0 Quality Control 161
10.0 Notes 162
11.0 References 162
Chapter 20 Determination of Bacterial Production using
Methyltritiated Thymidine
1.0 Scope and field of application 163
2.0 Definition 163
3.0 Principle of Analysis 163
4.0 Apparatus 163
5.0 Reagents 164
6.0 Sampling and incubation 165
7.0 Procedures 166
8.0 Calculation and expression of results 166
9.0 Quality Control 167
10.0 Interpretation of results 168
11.0 References 168
Chapter 21 Determination of Bacterial Production using
Tritiated Leucine
1.0 Scope and field of application 171
2.0 Definition 171
3.0 Principle of Analysis 171
4.0 Apparatus 172
5.0 Reagents 172
6.0 Sampling and incubation 173
7.0 Procedures 173
8.0 Calculation and expression of results 174
9.0 Other Remarks 175
10.0 References 176
Chapter 22 Microzooplankton Biomass
1.0 Scope and field of application 179
2.0 Definition 179
3.0 Principle 179
4.0 Apparatus 179
5.0 Reagents 180
6.0 Sampling 180
7.0 Procedures 180
8.0 Calculation and expression of results 182
9.0 Quality control and Assessment 183
10.0 Notes 183
11.0 Intercomparison 183
12.0 References &JGOFS papers published
using these techniques 183
Chapter 23 Microzooplankton Herbivory
1.0 Scope and field of application 185
2.0 Definition 185
3.0 Principle 185
4.0 Apparatus 186
5.0 Reagents 186
6.0 Sampling 186
7.0 Procedures 187
8.0 Calculation and expression of results 189
9.0 Quality control and assessment 190
10.0 Notes 190
11.0 Intercomparison 190
12.0 References & JGOFS papers published
using these techniques 190
Chapter 24 JGOFS Sediment Trap Methods
1.0 Introduction 193
2.0 Scope and field of application 193
3.0 Definition 193
4.0 Principle of Analysis 194
5.0 Apparatus 194
6.0 Reagents 194
7.0 Sampling 194
8.0 Post-collection Procedures 196
9.0 Calculation and expression of results 199
10.0 Quality Control/Quality Assessment 199
11.0 Intercomparison 200
12.0 Notes 200
13.0 References 200
Chapter 25 Trap-Collected Particle Flux with Surface-Tethered
Traps
1.0 Scope and field of application 203
2.0 Definition 203
3.0 Principle of Analysis 204
4.0 Apparatus 204
5.0 Reagents 205
6.0 Sampling 205
7.0 Sample Processing Procedures 206
8.0 Calculation and expression of results 207
9.0 Quality Control and Assessment 207
10.0 References 208
