JGOFS Protocols
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Protocols for the JGOFS core measurements (JGOFS Report Nr. 19)

Erratum document


Other titles in the JGOFS Report Series

In order to have a complete set of the JGOFS measurements protocols, you should get the DOE (1994) Handbook of methods for the analysis of the various parameters of the carbon dioxide system in sea water. Version 2, A. G. Dickson & C. Goyet, eds. ORNL/CDIAC-74DOE, prepared by the DOE Carbon Dioxide Survey Science Team. The handbook is online at the web site of the Carbon Dioxide Quality Control Program and printed copies are available on request from:

  Carbon Dioxide Information and Analysis Center
  Oak Ridge National Laboratory
  P.O. Box 2008
  Oak Ridge, Tennessee, USA
  E-mail: cdiac@ornl.gov

JGOFS Report Nr. 19 Table of Contents, also available in pdf format

Preface                                           	1
Chapter 1  Introduction by Dr. A.H. Knap          	3
Chapter 2  Shipboard Sampling Procedures
1.0 	Introduction                              	5
2.0	Hydrocasts                                	5
3.0	Water Sampling                            	6
4.0	Primary Production                        	6
5.0	Sediment Trap Deployment and Recovery     	7
6.0	Shipboard Sample Processing               	7
Chapter 3 CTD and Related Measurements
1.0	Scope and field of application			9
2.0 	Apparatus					9
3.0	Data Collection					11
4.0	Data Processing					12
5.0	References					16
Chapter 4 Quality Evaluation and Intercalibration
1.0	Introduction					19
Chapter 5 Salinity Determination
1.0	Scope and field of application			21
2.0	Definition					21
3.0	Principle of Analysis				21
4.0 	Apparatus					21
5.0	Reagents					21
6.0	Sampling					22
7.0	Procedures					22
8.0	Calculation and expression of results		22
9.0	Quality assurance				23
10.0	References					23
Chapter 6 Determination of Dissolved Oxygen by the Winkler
           Procedure
1.0	Scope and field of application			25
2.0	Definition					25
3.0	Principle of Analysis				25
4.0	Apparatus					26
5.0	Reagents					27
6.0	Sampling					28
7.0	Tiration Procedures				29
8.0	Calculation and expression of results		31
9.0	Quality assurance				32
10.0	References					33
Chapter 7 The Determination of Total Inorganic Carbon by
          the Coulometeric Procedure
1.0	Scope and field of application			35
2.0	Definition					35
3.0	Principle of Analysis				35
4.0	Apparatus					36
5.0	Reagents					37
6.0	Sampling					38
7.0	Procedures					39
8.0	Calculation and expression of results		41
9.0	Quality assurance				41
10.0	References					42
Chapter 8 The Determination of Nitrite, Nitrate +Nitrite,
          Orthophosphate and Reactive Silicate in Sewater
          using continuous Flow Analysis
1.0	Scope and field of application			43
2.0	Definition					43
3.0	Principle of Analysis				45
4.0	Apparatus					46
5.0	Reagents					51
6.0	Sampling					54
7.0	Procedures and Standardization			57
8.0 	Analytical Methods				65
9.0	Calculations					81
10.0	Quality Assurance				88
11.0	References					90
Chapter 9  The Determination of Nitrate in Sea Water
1.0	Scope and field of application			93
2.0	Definition					93
3.0	Principle of Analysis				93
4.0	Apparatus					94
5.0	Reagents					94
6.0	Sampling					94
7.0	Procedures					95
8.0	Calculation and expression of results		97
9.0	Notes						97
10.0	References					98
Chapter 10  The Determination of Nitrite in Sea Water
1.0	Scope and field of application			99
2.0	Definition					99
3.0	Principle of Analysis				99
4.0	Apparatus					99
5.0	Reagents					99
6.0	Sampling					100
7.0	Procedures					100
8.0	Calculation and expression of results		101
9.0	References					102

Chapter 11 The Determination of Phosphorus in Sea Water
1.0	Scope and field of application		 	103
2.0	Definition	  				103
3.0	Principle of Analysis	 	 		103
4.0	Apparatus					103
5.0	Reagents					103
6.0	Sampling					104
7.0	Procedures					104
8.0	Calculation and expression of results		105
9.0	References					106
Chapter 12 The Determination of Reactive Silicate in Sea
            Water
1.0	Scope and field of application			107
2.0	Definition					107
3.0	Principle of Analysis				107
4.0	Apparatus					107
5.0	Reagents					107
6.0	Sampling					108
7.0	Procedures					108
8.0	Calculation and expression of results		109
9.0	Notes						110
10.0	References					110
Chapter 13  Measurement of Algal Chlorophylls and Carotenoids
	by HPLC
1.0	Scope and field of application			111
2.0	Definition					111
3.0	Principle of Analysis				111
4.0	Apparatus and Reagents				112
5.0	Eluants						113
6.0	Sample Collection and Storage			113
7.0	Procedure					113
8.0	Calculation and expression of results		115
9.0	References					115
Chapter 14  Measurement of Chlorophyll a and Phaeopigments
	by Fluorometric Analysis
1.0	Scope and field of application			119
2.0	Definition					119
3.0	Principle of Analysis				119
4.0	Apparatus					119
5.0	Reagents					120
6.0	Sample Collection and Storage			120
7.0	Procedure					120
8.0	Calculation and expression of results		122
9.0	References					122
Chapter 15  Determination of Particulate Organic Carbon and
	 Particulate Nitrogen
1.0	Scope and field of application			123
2.0	Definition					123
3.0	Principle of Analysis				123
4.0	Apparatus					123
5.0	Reagents					124
6.0	Sampling					124
7.0	Procedures					124
8.0	Calculation and expression of results		125
9.0	References					125
Chapter 16  Determination of Dissolved Organic Carbon by a
            High Temperature Combustion/Direct Injection
            Technique
1.0	Scope and field of application			127
2.0	Definition					127
3.0	Principle of Analysis				127
4.0	Apparatus					128
5.0	Reagents					129
6.0	Sampling					130
7.0	Procedures					131
8.0	Calculation and expression of results		134
9.0	Quality control/quality assessment		137
10.0	Notes						139
11.0	Intercomparison					141
12.0	References					142
Chapter 17  Determination of New Production by 15N
1.0	Scope and field of application			145
2.0	Definition					145
3.0	Principle of Analysis				145
4.0	Apparatus					145
5.0	Reagents					146
6.0	Sampling					146
7.0	Procedures					146
8.0	Calculation and expression of results		147
9.0	Quality Control					148
10.0	Intercomparison					149
11.0	Parameters					149
12.0	References					149
Chapter 18  Determination of Bacterioplankton Abundance
1.0	Scope and field of application			151
2.0	Definition					151
3.0	Principle of Analysis				151
4.0	Apparatus					151
5.0	Reagents					152
6.0	Sampling					152
7.0	Procedures					152
8.0	Calculation and expression of results		153
9.0	Quality Control					153
10.0	References					154
Chapter 19  Primary Production by 14C
1.0	Scope and field of application			155
2.0	Definition					155
3.0	Principle of Analysis				155
4.0	Apparatus					156
5.0	Reagents and Supplies				156
6.0	Sampling					158
7.0	Procedures					160
8.0	Calculation and expression of results		160
9.0	Quality Control					161
10.0	Notes						162
11.0 	References					162
Chapter 20  Determination of Bacterial Production using
	Methyltritiated Thymidine
1.0	Scope and field of application			163
2.0	Definition					163
3.0	Principle of Analysis				163
4.0	Apparatus					163
5.0	Reagents					164
6.0	Sampling and incubation				165
7.0	Procedures					166
8.0	Calculation and expression of results		166
9.0	Quality Control					167
10.0	Interpretation of results			168
11.0 	References					168
Chapter 21  Determination of Bacterial Production using
            Tritiated Leucine
1.0	Scope and field of application			171
2.0	Definition					171
3.0	Principle of Analysis				171
4.0	Apparatus					172
5.0	Reagents					172
6.0	Sampling and incubation				173
7.0	Procedures					173
8.0	Calculation and expression of results		174
9.0	Other  Remarks					175
10.0	References					176
Chapter 22  Microzooplankton Biomass
1.0	Scope and field of application			179
2.0	Definition					179
3.0	Principle 					179
4.0	Apparatus					179
5.0	Reagents					180
6.0	Sampling					180
7.0	Procedures					180
8.0	Calculation and expression of results		182
9.0	Quality control and Assessment			183
10.0	Notes						183
11.0	Intercomparison					183
12.0 	References &JGOFS papers published
        using these techniques				183
Chapter 23  Microzooplankton Herbivory
1.0	Scope and field of application			185
2.0	Definition					185
3.0	Principle 					185
4.0	Apparatus					186
5.0	Reagents					186
6.0	Sampling					186
7.0	Procedures					187
8.0	Calculation and expression of results		189
9.0	Quality control and assessment			190
10.0	Notes						190
11.0	Intercomparison					190
12.0 	References & JGOFS papers published
        using these techniques				190
Chapter 24  JGOFS Sediment Trap Methods
1.0	Introduction					193
2.0	Scope and field of application			193
3.0	Definition					193
4.0	Principle of Analysis				194
5.0	Apparatus					194
6.0	Reagents					194
7.0	Sampling					194
8.0	Post-collection Procedures			196
9.0	Calculation and expression of results		199
10.0	Quality Control/Quality Assessment		199
11.0	Intercomparison					200
12.0 	Notes						200
13.0 	References					200
Chapter 25  Trap-Collected Particle Flux with Surface-Tethered
            Traps
1.0	Scope and field of application			203
2.0	Definition					203
3.0	Principle of Analysis				204
4.0	Apparatus					204
5.0	Reagents					205
6.0	Sampling					205
7.0	Sample Processing Procedures			206
8.0	Calculation and expression of results		207
9.0	Quality Control and Assessment			207
10.0	References					208