Appendix I

UPDATED DRAFT AGENDA

BATS Oversight Committee Meeting, March 23-25, 1996

SATURDAY 23 March

0800 Breakfast

0900 Introductory Discussion re-evaluation of agenda and logistics

BATS program and science overview and ancillary programs

Bermuda Testbed Mooring Program (Dickey)

Tour of ship and mooring

1200 Lunch

1330 Lab tour (1 hour)

DIC/Alk science and methoda (Bates, 1.5 hr)

DOC/N science and methods (Hansell, Carlson, 1.5 hr)

Particle fluxes science and methods (Michaels, 1 hr)

1930 Dinner in town

SUNDAY 24 March

0900 BATS data, distribution and use

BATS data system on site and over the Web

Existing sunthesis plans and people

1200 Lunch

1330 The future: (4-5 hrs)

Forward looking science issues

Missing methods (mets)

Integration of ancillary measurements

3-D issues

Moorings and remote sensing

etc.

1930 Dinner (committee only)

MONDAY 25 March

0800 Breakfast

Finish up loose ends (meeting just with PIs?)

Transition to post-JGOFS world

1200 Lunch

Identification of tasks required to complete the report

Mid afternoon adjourn

Appendix II

JGOFS Time-series Oversight Committee Meeting

29-31 May 1996

Marine Science Bldg. #315

University of Hawaii, Honolulu, Hawaii

AGENDA

(draft 5/15/96)

WEDNESDAY, 29 May

0800 hr: pick up at Kaimana Beach Hotel (if transportation is required)

0815-0830 hr: coffee/manapuas

0830-0915 hr: Introduction and logistics (Dave Karl)

Review of agenda (J. McCarthy/D. Karl)

Role of T-S oversight committee and objectives of site visit (J. McCarthy)

0915-1030 hr: HOT program overview (D. Karl)

design sampling strategies, core measurement list, ancillary investigators

major scientific accomplishments to date

- closure of upper water column C cycle

- importance of N2 fixation and nitrification in upper water column

- P vs. N limitation and possible basin-scale effects

- detection of the Hawaiian Ridge current

- deep, cold water surges

- hypothesis generation and testing

- marine science education

1030-1045 hr: break

1045-1200 hr: HOT program protocols with focus on "problem" areas and on QA/QC

1200-1300 hr: lunch at UH Campus Center or Manoa Gardens

1300-1500 hr: HOT-BATS measurement intercomparisons with focus on differences in measurement protocols and differences in measurement needs (i.e., Atlantic vs. Pacific)

1500-1515 hr: break

1515-1600 hr: Continue discussions on core measurement protocols and intercomparisons

1600-1730 hr: tour of HOT-Marine Scince building facilities in WOCE and JGOFS programs: shops, laboratories and computer facilities (we will probably break up into 2 or 3 smaller groups for these tours)

1730-??? hr: return to hotel or (better yet) join in at Aloha Tower happy hour at our local microbrewery and stay for dinner at one of several eateries (city-operated trams are available for transportation back to Waikiki at regular intervals and some of us will also be available to provide transportation

THURSDAY, 30 May

Early AM

(0500-0830 hr): free for a swim in the Pacific, a jog in Kapiolani Park or a climb to the summit of Diamond Head crater

0900 hr: pick up at hotel, drive to U.H. Marine Expeditionary Center (Snug Harbor)

0930-1130 hr: tours of HOT program field staging laboratories, the R/V Moana Wave and R/V K-O-K, and other Snug Harbor facilities

1130 hr: lunch on R/V Moana Wave

1300-1500 hr: Data management and data availability issues

1500-1515 hr: guava juice and papaya break

1515-1730 hr: Continue data management issues, including demonstration of HOTDOGS

1730 hr: Return to hotel for a swim or jog, rent a boogie board at Queen's Surf or a kayak at the hotel, hit the bars for a "stiff one" or whatever

1900 hr: restaurant lists available for excellent Thai, Filipino, Chinese, Japanese or other cuisines - HOT program staff will organize and host ad hoc dinner groups as desired (e.g., one option is to descend, en masse on Mekong I, one of Honolulu's best Thai restaurants).

FRIDAY, 31 MAY

0830-1200 hr: Mid-term adjustments and longer term future for JGOFS (and WOCE) oceanic time- series stations

- revised scientific/ecosystem models

- new measurements and measurement techniques

- remote sensing and novel instrumentation

- new funding paradigms (life after WOCE and JGOFS)

- 3D issues and horizontal advection

1145-1300 hr: bento lunch at Washila Ridge State Park

1300-1515 hr: "closed-door" Oversight Committee meeting

1515-1530 hr: break

1530-1700: Oversight Committee meeting with HOT asnd BATS program P.I.s

1730 hr: SOEST T.G.I.F. - meet old friends and make a few new ones

PM on your own for a night on the town or "red-eye" airline departures

Appendix III

Pigment Intercalibration Exercises at the HOT and BATS Time-series Stations

June 1996

R.R. Bidigare and M. Latasa

Department of Oceanography

University of Hawaii

Honolulu, HI 96822

The initial results of a HOT-BATS HPLC pigment intercalibration were presented by Tony Michaels at the most recent oversight committee meeting in Honolulu (May, 1996). The results suggest that there is a ca. 2-fold difference in all pigment concentrations determined for replicate filters (=natural samples collected at the BATS site) analyzed by the HOT and BATS pigment groups. This difference could have been explained by one or more of the following mechanisms (and the first four have been ruled out by the analyses presented below):

(1) an error in the calculation of pigment concentrations;

(2) the spectrophotometers used to calibrate the HPLC pigment standards at the HOT and BATS pigment labs differ by a factor of two;

(3) the HPLC systems used to measure pigment concentrations at the HOT and BATS pigment labs differ by a factor of two;

(4) differences in the pigment extraction efficiencies obtained by the HOT and BATS pigment labs; and

(5) the filters analyzed by the HOT and BATS pigment groups were different (e.g., breakdown of pigments during shipment to Hawaii, different volumes of seawater were used to prepare the "replicate" filters, etc.).

The pigment calculations by both labs were carefully checked and found to be correct, thus eliminating the first explanation. To address the second and third explanations, both HOT and BATS pigment labs analyzed pure pigment standards by spectrophotometry and HPLC. These standards were made available through a NASA-sponsored SeaWiFS project and included monovinyl chlorophyll a, fucoxanthin, diadinoxanthin, zeaxanthin and peridinin. A comparison of results is given in the attached tables andfigures. Spectrophotometric analyses agreed to within 1% to 6%, depending on which pigment is compared (see Figure 1 and Table 1). The HPLC measurements using existing calibration factors agreed to within 2% to -27%, with an average of 15% lower for BATS (see Figure 2 and Table 2). We conclude that the 2-fold difference observed for the replicate filter analyses was not caused by the second and third explanations given above. Furthermore, since the extraction techniques used by the HOT and BATS pigment labs are identical, we rule out the possibility of the fourth explanation. Thus, it is likely that the 2-fold differences observed in the initial comparison resulted from the differences in the filters analyzed.

The pigment standards were also used to calculate new calibration facters. For HOT, the old and new calibration factors were similar (<10% difference) for all pigments except diadinoxanthin (Table 3). With the exception of chlorophyll a, the BATS old and new calibration factors differed by 20% (Table 4). In all cases, a good agreement (4%) was observed for the determination of chlorophyll a concentration. This result is not surprising since, in contrast to accessory pigments, chlorophyll a is readily available and routinely used for instrument calibration. Until recently, accessory pigment standards have not been readily available for routine instrument calibration (see attached VKI Water Quality Institute brochure). Thus, the poorer agreement for the accessory pigments shown in Table 2 probably results from infrequent HPLC calibration for these pigments. We will continue to supply the BATS pigment lab with NASA standards to ensure interlaboratory agreements for accessory pigment quantification. Future intercalibration exercises will include the analysis of:

(1) pure pigment standards, including chlorophyll a;

(2) pigment extracts prepared from the same filter; and

(3) replicate filters collected at the HOT and BATS time-series stations.