PI: Walker Smith of: Virginia Institute of Marine Science dataset: Nano- and microplankton abundance and carbon biomass project/cruise: AESOPS/NBP-96-4A, Ross Sea Process Cruise 1 and AESOPS/NBP-97-1, Ross Sea Process Cruise 2 ship: Nathaniel B. Palmer
Whole water samples were preserved with acidic Lugol's iodine solution and/or glutaraldehyde-Lugol's solution (35 o/oo, v/v) at a final concentration of 1% (Rousseau et al., 1990). Diatoms and plankton bigger than 20 micron (including larger dinoflagellates of known trophic mode and most of the ciliates) were enumerated from 50 or 100 ml of these samples that were settled and counted with an inverted microscope (Utermöhl, 1958). Phaeocystis antarctica colonies were enumerated and measured in these samples, and colonial cell abundance was calculated from colony volume according to Mathot et al. (subm.).
Biomass was estimated by converting cell volumes (calculated from measurements of cell dimensions and using volume equations for appropriate geometric shapes) using the relationships [log10 C = 0.94 (log10 V) - 0. 60] and [log10 C = 0.76 (log10 V) - 0. 352] for flagellates and diatoms respectively (with C representing carbon as picograms per cell and V representing cell volume in cubic microns; Eppley et al., 1970). For ciliates, conversion factors outlined by Stoecker et al. (1994) were used.
Booth, B.C. 1993. Estimating cell concentration and biomass of autotrophic plankton using microscopy. In: Handbook of Methods in Aquatic Microbial Ecology. P.F. Kemp, B.F. Sherr, E.B. Sherr, and J.J. Cole (eds.), Lewis Publ., Boca Raton. Pp. 199-205.
Coleman, A.W. 1980. Enhanced staining of bacteria in natural environments by fluorochrome staining of DNA. Limnol. Oceanogr. 25: 948-951.
Eppley, R.W., F.M.H. Reid and J.D.H. Strickland. 1970. The ecology of the plankton off La Jolla, California, in the period April through September 1967; Part III. Estimates of phytoplankton crop size, growth rate and primary production. Bull. Scripps Inst. Oceanogr. 17: 33-42.
Mathot S., W.O. Smith, Jr., C.A. Carlson, D.L. Garrison & M.M. Gowing Estimation of Phaeocystis antarctica (Prymnesiophyceae) carbon biomass in the Ross Sea, Antarctica. J. Phycol. (subm.)
Rousseau, V., S. Mathot and C. Lancelot. 1990. Calculating carbon biomass of Phaeocystis sp. from microscopic observations. Mar. Biol. 107: 305-314.
Stoecker, D.K., D.J. Gifford and M. Putt. 1994. Preservation of marine planktonic ciliates: losses and cell shrinkage during fixation. Mar. Ecol. Prog. Ser. 110: 293-299.
Utermöhl, H. 1958. Zur Vervollkommnung der quantitativen Phytoplankton-Methodik. Mitt. int. Verein. theor. angew. Limnol. 9: 1-38.