PI:       Michael R. Landry
of:       University of Hawaii
dataset:  Abundances of picoplankton, phytoplankton and bacteria by flow cytometry

Methods:

Heterotrophic bacteria and Synechococcus abundances were determined using small
volume flow cytometry (SV FCM), whereas Eukaryotic Phytoplankton abundances
were determined using large volume flow cytometry (LV FCM).

SV FCM:
These flow cytometry samples (1 ml volume) were preserved with 0.5%
paraformaldehyde, liquid nitrogen frozen and analyzed in batches on land for
prokaryotic abundances (Synechococcus and heterotrophic bacteria).  They were
analyzed using a Coulter EPICS 753 flow cytometer with two lasers (UV and 488
nm) using the protocol of Monger and Landry (Flow cytometric analysis of marine
bacteria with Hoechst 33342, Appl. Environ. Microbiol. 59:905-911, 1993).

LV FCM:
These flow cytometry samples were run within 1-2 h of collection, without
preservative. The shipboard flow cytometer was a Coulter EPICS Profile II with
an argon air-cooled laser (35 mW), a biosense flow cell, and it was modified to
handle sample volumes up to 60 ml and delivery rates up to 3 ml min^-1.