PI: Michael R. Landry of: University of Hawaii dataset: Abundances of picoplankton, phytoplankton and bacteria by flow cytometry Methods: Heterotrophic bacteria and Synechococcus abundances were determined using small volume flow cytometry (SV FCM), whereas Eukaryotic Phytoplankton abundances were determined using large volume flow cytometry (LV FCM). SV FCM: These flow cytometry samples (1 ml volume) were preserved with 0.5% paraformaldehyde, liquid nitrogen frozen and analyzed in batches on land for prokaryotic abundances (Synechococcus and heterotrophic bacteria). They were analyzed using a Coulter EPICS 753 flow cytometer with two lasers (UV and 488 nm) using the protocol of Monger and Landry (Flow cytometric analysis of marine bacteria with Hoechst 33342, Appl. Environ. Microbiol. 59:905-911, 1993). LV FCM: These flow cytometry samples were run within 1-2 h of collection, without preservative. The shipboard flow cytometer was a Coulter EPICS Profile II with an argon air-cooled laser (35 mW), a biosense flow cell, and it was modified to handle sample volumes up to 60 ml and delivery rates up to 3 ml min^-1.