PI: Michael R. Landry of: University of Hawaii dataset: Nano- and microplankton abundances by epifluorescence microscopyMethodology:
The epi-slide counts were made from 50 ml or 250 ml samples filtered onto 1 um or 10 um filters, respectively, and mounted on slides. These slides were imaged within 1 - 2 d of collection, then images were analyzed on land for eukaryotic nano and microplankton abundances and biomass The 50 ml samples were used to estimate nanoplankton (1.5 to 20 um) abundances and biomass, whereas the 250 ml samples were used to estimate microplantkon (>20 um) abundances and biomass. Cell volume (calculated using the length and width and assuming a prolate spheroid shape) was converted to biomass using the modified Strathman equations. References: Strathman, R.R. Estimating the organic carbon content of phytoplankton from cell volume or plasma volume, Limnol. Oceanogr. 36:50-63, 1967. Eppley, R.W., F.M.H. Reid, and J.D.H. Strickland, Estimates of phytoplankton crop size, growth rate, and primary production, IN: J.D.H. Strickland (Ed.), The ecology of the plankton off La Jolla California in the period April through September, 1967, Bulletin ofScripps Institution of Oceanography, La Jolla, CA, 17:33-42, 1970.